Plasmid

Part:BBa_K5226078:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-10-01)


321（C+cysNC）-194-4hbd-sucD-ogdA-194-orfZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2528
    Illegal BamHI site found at 6605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2981
    Illegal NgoMIV site found at 3866
    Illegal NgoMIV site found at 4977
    Illegal NgoMIV site found at 5101
    Illegal AgeI site found at 2037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2103
    Illegal SapI.rc site found at 2171
    Illegal SapI.rc site found at 10221


Design Notes

none


Source

The pSEVA321 backbone and gene cluster is directly obtained from the laboratory.CysNC and its RBS are obtained by PCR amplification.Sequence source is from Halomonas sp. TD01 strain, found through NCBI(https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=2677331&to=2679235)

References